Development of cost-effective tetra-ARMS PCR for detection of FecB genotype in sheep*
نویسندگان
چکیده
1 College of Life Sciences, China Jiliang University, Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Institute of Plant Environmental Biology and Measurement Technology, Hangzhou 310018, China 2 The Key Sheep Breeding and Reproduction Biotechnology Laboratory of Xinjiang Production and Construction Group, Research Institute of Animal Husbandry and Veterinary, Xinjiang Agricultural Reclamation Academy, Shihezi 832000, China
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Detection of Single-Nucleotide Polymorphism in the Bovine AGPAT6 Gene Associated with Milk Fat Content using Tetra-Primer ARMS PCR-based Assay in Karan Fries Breeding Bulls
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Sixty eight adult ewes of Zel sheep breed were screened with forced PCR RFLP method for the detection of the FecB gene and its effects on litter size. Genomic DNA was extracted from the blood of 68 Zel matured ewes with litter size varied from 1 to 2 lambs per ewe lambing. Digestion of the FecB gene (190 base pairs) with AvaII restriction enzyme resulted in none carrier 190 bp band wild type in...
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